Overtraining on Renal Tissue Were Simulated under Microscope for Ultrapathology and Enzyme Histochemistry

  • Bing Zhou
Keywords: Simulated Overtraining, Renal Tissue Injury, Transmission Electron Microscope, Urine Index, Enzyme Activity


To study the pathological changes of renal tissue under the condition of overloading exercise in rats by
establishing a motor model, and to explore the possible mechanism of renal tissue injury caused by overtraining,
so as to provide a new idea for the prevention and treatment of renal tissue injury caused by overtraining. 60 rats
were used to establish the disposable exhaustion model or continuous training model, and the control group rat
model was established respectively. The experimental group rats were only observed by histological
transmission electron microscopy and enzyme histochemical observation. Rats in the experimental group were
trained at one time and at the same time. Each experiment had 15 samples, and the corresponding control group
also had 15 samples. The urine index and the activity of Na+, K+-ATP in the renal tissue of the rats in the
disposable exhaustion group were observed immediately after the training and 24 hours later, respectively. Urine
index and Na+,K+ -atpase activity in renal tissue were measured by the continuous exhaustion training team at
week 4 and 8 of the training. It was found that the urinary protein and urinary NAG enzyme of the rats were
significantly higher than those of the control rats immediately after the training (P<0.05), and the activities of
Na+ ,K+-ATP in the renal tissue were significantly reduced (P<0.05), and recovered after 24 hours, but still lower
than those of the control rats. The activities of urinary protein, urinary NAG enzyme and renal tissue
Na+ ,K+-ATP were significantly increased after 4 weeks of continuous exhaustion training in rats compared with
the control group (P<0.05). After 8 weeks of training, urinary protein and urinary NAG enzyme were
significantly increased compared with the control group, while the activities of renal tissue Na+ ,K+-ATP were
significantly decreased (P<0.05), and were negatively correlated with the excretion of urinary NAG enzyme.
Continuous overtraining can cause significant changes in ultrastructure of renal tissue and damage to renal