Melatonin on the Ultrastructure of Liver and Kidney Cells after an Exhausting Exercise in Sprint Training under Electron Microscope

  • Shanshan Xia
Keywords: Sprint Training, Endurance Training, Exhaustive Exercise, Liver and Kidney Ultrastructure


A lot of research shows. Exercise, especially exhausting exercise, can produce a lot of exercise-induced free
radicals. There is a high affinity between free radicals and unsaturated fatty acids in biofilm. Once the two are
combined, they will form an unsaturated fatty acid peroxide. At the same time, free radicals can also oxidize and
destroy membrane protein components and many components in cells. Based on the above background, the
purpose of this paper is to study the effect of melatonin on the ultrastructure of liver and kidney cells after an
exhaustive exercise. In this experiment, mice were taken as the experimental objects, endurance training was
carried out with increasing intensity, and an exhaustive swimming model was established. SOD activity was
determined by pyrogallol autoxidation method, CAT activity by ultraviolet spectrophotometry, POD activity by
guaiacol method, MDA content by TBA method, got and GPT activity in serum by King Di colorimetry, Hb
content by cyaniding high iron method, glycogen content by anthrone method, ultrastructural changes of
hepatocytes by transmission electron microscope. The results showed that endurance training and MT
supplementation could significantly prolong the time of exhaustive swimming in mice (P < 0.01), and
exhaustive exercise could significantly damage the ultrastructure of liver and kidney, while the damage of liver
and kidney in mice taking MT was less.