Soybean Isoflavones on the Morphology and Ultrastructure of Hippocampus in Alzheimer's disease Model Rats Induced by Aβ25~35

  • Hui Zhou
Keywords: Soybean Isoflavone, Alzheimer Disease, Hippocampus Research, Ultrastructure Research


In recent years, the number of AD patients has shown a gradual upward trend globally. Soy isoflavones have the
effects of enhancing immunity and weak estrogen-like, antioxidant and cut cancer cell growth. Therefore, the
purpose of this article is to study the effect of soybean isoflavones on the morphology and ultrastructure of
hippocampus in Alzheimer's disease (AD) model rats induced by Aβ25-35. The method in this paper is to use
experimental comparison method to randomly divide 60 experimental rats into model control group, sham
operation group, Aβ+ low-dose soybean isoflavone group (26.3mg•kg-1•d-1), Aβ+ High-dose soybean
isoflavones (52.6mg•kg-1•d-1) and Aβ+estrogen group were injected with Aβ25-35 bilateral hippocampus. By
adjusting different doses of soybean isoflavones, bioactive molecules were extracted for HE staining.
Subsequently, the effect of soybean isoflavones on the morphology and ultrastructure of hippocampus was
observed by electron microscope. Through analysis and determination, it can be found that in the model group,
the number of neurons in the hippocampal CA1-CA4 area and dentate gyrus of the rat is significantly reduced
by 24%, and there are many cytoplasmic staining of nerve cells. In the three groups of the experimental group,
the number of neurons increased compared with the model group and the sham operation group, and the number
of cytoplasmic stained neurons was relatively small. Compared with the model control group, the neurons were
less polluted by 21%, but some nerves were present Phenomenon such as cell membrane shrinkage, the nuclear
membrane as a whole is relatively complete, the model group has fewer neurons in the hippocampal CA1 area,
and the cell body shrinks. According to the data comparison, the corresponding conclusion is that the abnormal
neurons in the hippocampal CA1 area ofthe soybean isoflavone-related treatment group are significantly
reduced compared with the model group, which can effectively improve the loss of hippocampal neurons in AD
rats caused by Aβ25-35 and ensure normal tissue morphology and structure.