MiR-125b-5p Promotes Autophagy in Systemic Lupus Erythematosus Cells by Up Regulating the Expression of Genes Related to UV Resistance

  • Lei Liu
Keywords: Systemic Lupus Erythematosus, Mir-125b-5p Gene, Ultraviolet Irradiation, Autophagy

Abstract

To investigate the effect of mir-125b-5p on autophagy of SLE cells by up regulating the expression of UV
resistance related genes. Systemic lupus erythematosus (SLE) model was used. The SD rats were half male and
half female, 170-200g. 60 rats provided by the Institute were divided into two groups, with an average of 30 rats
in each group. In the non irradiation group, the supernatant was centrifuged directly and added into RPMI1640
complete medium, then put into cell incubator for further culture. After 24 hours of irradiation, the cells were
centrifuged for subsequent detection. To observe the effect of ultraviolet on autophagy, protein and mRNA
expression, and to analyze the data by logic analysis. Compared with the control group (omj / cm2), 12.5mj/cm2
and 200mj / cm2, the number of autophagy of rfp-lc3 in 50mj / cm2 group was the highest at 12h, and the
difference was statistically significant. At 24 hours, compared with other dose groups, the number of autophagy
in 50 MJ / cm2 group was the highest (P > 0.01). There was no significant difference among the other groups.
Compared with the blank control group (omj / cm2) and other dose response groups, the expression level of
beclin-1 and lc3ii protein in the 50mj / cm2 dose group was the highest, but only beclin-1 had statistical
significance. The expression level of beclin-1 mRNA in 50mj / cm2 group reached the highest level in 24 hours.
Mir-125b-5p can significantly promote the autophagy of SLE cells by up regulating the expression of anti UV
related genes. Autophagy plays an important role in maintaining the homeostasis and function of immune cells
and immune system.

Published
2020-06-01