Effect of Growth Hormone on Clara Cells in Rats with Acute Lung Injury

  • Zhifeng Jiang
Keywords: Growth Hormone Acute Lung Injury Clara Cells Cytokines Research

Abstract

To investigate the effect of growth hormone on Clara cells in rats with acute lung injury. Healthy male rats aged
6 8 weeks and weighing 18 22g were selected. They were randomly divided into normal control group (NS
group), sham operation group (sham group ), CLP Group and growth hormone intervention group. NS group:
normal saline was injected intraperitoneally. Sham operation group: intraperitoneal injection of 1%
Pentobarbital, fixed after anesthesia. Under aseptic condition, incise along the midline of ab domen, find the
cecum, and seal the abdomen layer by layer. The animal model of acute lung injury induced by CLP was
established. The animals were fed adaptively for 1 week. Lung water content, serum inflammatory mediators,
survival rate, t PA and PAI 1 le vels were observed before the experiment. The data were statistically analyzed
by logical analysis. In terms of lung water content, the lung water content of each growth hormone intervention
group was lower than that of CLP Group (P< 0.001); the w / D ratio of rats was significantly increased (P< 0.001),
indicating that pulmonary edema was aggravated. There was significant difference between growth hormone
intervention group and CLP Group (P< 0.001). In serum inflammatory mediators, serum IL 6 and IL 1 protein
were significantly increased in CLP Group. Compared with CLP Group, the survival time of GH intervention
group C was significantly prolonged, and the survival rate was si gnificantly increased by 20% (P< 0.05). The
survival rate of growth hormone interventi on group was significantly improved. At the levels of t PA and PAI 1,
the ratio of t PA / P AI 1 increased significantly (P< 0.001), which was related to the increase of fibrinolytic
activity by growth hormone. GH has anti inflammatory and pulmonary vascular permeability, and can
significantly improve the survival rate of CLP rats.

Published
2020-06-01