Effects of Growth Hormone Pretreatment on Apoptosis and Energy Metabolism after Myocardial Ischemia-Reperfusion in Rats

  • Qingrong Cheng
Keywords: Growth Hormone Pretreatment, Rats, Myocardial Ischemia-Reperfusion, Apoptosis, Energy Metabolism

Abstract

To explore the effect of growth hormone pretreatment on apoptosis and energy metabolism after myocardial
ischemia-reperfusion in rats, and analyze its mechanism. 60 rats were randomly divided into two groups, 30 rats
in each group. The control group was injected with normal saline, and the observation group was pretreated with
growth hormone, 5mg / kg. The degree of apoptosis, the content of superoxide dismutase (SOD) and glutathione
(GSH), the expression level of bcl-1 and IGF-1 protein related to apoptosis, and the expression level of ERK
and Juk protein related to energy metabolism were compared. Compared with the control group, the apoptosis of
the observation group was significantly reduced, the difference was statistically significant (P < 0.05).
Compared with the control group, the content of superoxide dismutase (SOD) and glutathione (GSH) in the
serum of the observation group increased significantly (P < 0.05). Compared with the control group, the
expression of bcl-1 and IGF-1 protein in the observation group increased significantly (P < 0.05). Compared
with the control group, the expression level of energy metabolism related ERK and Juk protein in the
observation group increased significantly (P < 0.05). Growth hormone pretreatment can reduce apoptosis,
decrease energy metabolism, increase oxidative response, and protect cardiomyocytes through related protein
pathway.

Published
2020-05-01