Effects of Polysaccharide from Polygonatum Cyrtonema Hua on Blood Glucose, Survival Rate and Liver Cell Protection in Type I Diabetic Mice

  • Yi Lu
Keywords: Polysaccharide from Polygonatum cyrtonema Hua, Type I Diabetes Mellitus, Blood Glucose Level, Survival Rate, Liver Cell Protection

Abstract

To investigate the effects of Polygonatum cyrtonema Hua polysaccharide on blood glucose, survival rate and
liver protection in type 1 diabetic mice. A female mouse model of type 1 diabetes mellitus was established by
continuous intraperitoneal injection of streptozotocin. The experiment was mainly divided into four groups:
high-dose group, low-dose group, model group and blank control group. The injection dose of high-dose group
was 900 mg/kg, and that of low-dose group was 450 mg/kg. The blood glucose, survival rate and body weight of
mice in each group were closely monitored during the experiment. After the experiment, the liver of mice was
separated and the pathological changes of the liver were evaluated by HE staining. The levels of insulin receptor
substrate 1 mRNA, interleukin-1beta and interleukin-6 in liver were detected by quantitative PCR. Female mice
were intraperitoneally injected with streptozotocin for 5 consecutive days, and their blood glucose levels were
significantly increased after 7 days of treatment. Under the condition of hyperglycemia, the mortality of mice in
the model group will be significantly increased, and the high-dose treatment of Polygonatum cyrtonema Hua
polysaccharide can promote the survival rate of model mice significantly improved; Low and high doses of
Polygonatum cyrtonema Hua polysaccharide can significantly increase the body weight of model mice, and high
doses of Polygonatum cyrtonema Hua polysaccharide treatment can promote the reduction of blood glucose
levels. By HE staining, the results showed that the liver of model mice had obvious lesions: there were many
infiltration of mononuclear inflammatory cells, which significantly changed the nature of hepatocytes. At the
same time, hepatocytes develop structural disorders. However, the high-dose treatment of Polygonatum
cyrtonema Hua polysaccharide can significantly improve the liver structural disorders, hepatocyte degeneration
and active hepatocyte infiltration in the model mice. Quantitative PCR showed that polysaccharide from
Polygonatum cyrtonema Huacould decrease the levels of interleukin-1beta and interleukin-6 in diabetic liver,
and increase the level of insulin receptor substrate 1 mRNA significantly. Polygonatum cyrtonema Hua
polysaccharide can significantly reduce the mortality of type 1 diabetic mice. This may have a very close
relationship with its related factors, such as liver protection, blood glucose reduction and improving the weight
of mice.

Published
2020-05-01