Effects of Recombinant Human Erythropoietin on Angiogenesis and Neurological Function Recovery in Mice with Traumatic Brain Injury

  • Yezi Xia
Keywords: Human Erythropoiesis, Mice, Traumatic Brain Injury, Nerve Function


This paper investigates the influence of recombinant human erythropoietin on angiogenesis and neurological
function recovery in mice with traumatic brain damage. Flow cytometry with CD34- and CD133 particular
antibodies was used to observe variations in endothelial progenitor cells (EPC) level. To measure angiogenesis,
brain tissue sections contaminated with CD31 and CD34 antibodies can be used. The behavioral regaining is
evaluated by the Water Maze (MWM) test and the modified Neurological Severity Score (mNSS) test. In
comparison with the saline treatment, rhEPO treatment considerably increased the proportion of circulating
EPCs on days 1, 7, and 14 (P <0.05), and enhanced spatial learning ability on day 25 (P <0.05), and 26 days (P
<0.05) it also improved memory recovery. Moreover, the mNSS evaluation score on days 14, 21, and 25 (P
<0.05) was decreased by rhEPO treatment. Circulating EPC levels and CD34- and CD31 positive cells between
the injured border area (CD34 + r = 0.910, P <0.01; CD31 + r = 0.894, P <0.01) and the ipsilateral hippocampus
(CD34 + r =). There is a strong connection between: 0.841, P <0.01; CD31 + r = 0.835, P <0.01 rhEPO therapy
can ameliorate the nerve function recovery as well as it can enhance the angiogenesis of mice after TBI by
improving EPC.