The Effect of Adiponectin on Colorectal Cancer in Diabetic Mice and Its Target and Molecular Mechanism

  • Lixian Cheng
Keywords: Adiponectin, Diabetic Mice, Colorectal Cancer, Target, Molecular Mechanism

Abstract

To investigate the effect of adiponectin on colorectal cancer in diabetic mice, and analyze its target and
molecular mechanism. 60 diabetic mice were randomly divided into two groups, 30 in each group. The control
group was injected with normal saline, and the observation group was injected with adiponectin 5mg / kg. 12
weeks later, the tumor volume and blood glucose concentration of the two groups were compared; blood and
tumor tissue samples were collected before execution, the number of metastatic nodules was counted, and the
levels of adiponectin, leptin, IGF-1 and insulin were detected by ELISA. The expression levels of IGF-2R, RAS,
c-myc, β - Catenin, PPAR γ and PAK-1 were compared. Compared with the control group, the tumor volume,
number of nodules and blood glucose in the observation group were significantly reduced, the difference was
statistically significant (P < 0.05). Compared with the control group, the expression levels of leptin, IGF-1,
insulin, TNF - α, IL-6, IL-1 β protein in the observation group were significantly reduced (P < 0.05). Compared
with the control group, the expression levels of IGF-2R, RAS, c-myc, β - Catenin, PPAR γ and PAK-1 in the
observation group were significantly reduced (P < 0.05). Adiponectin has a very good therapeutic effect on
colorectal cancer in diabetic mice. It can inhibit the proliferation and metastasis of tumor, participate in the
apoptosis of tumor cells, improve insulin resistance, and has a strong correlation with the main tumor marker
proteins. It acts on tumor related signal pathways, providing new experimental data and evidence for the
prevention and treatment of colorectal cancer.

Published
2020-06-25