Ultrastructural Characteristics of Tympanic Sclerosis Guinea Pig Middle Ear Mucosa under Electron Microscope

  • Zhen Xu
Keywords: Tympanic Sclerosis, Middle Ear Mucosa, Ultrastructural Features, Guinea Pig

Abstract

Tympanic sclerosis is widespread in the middle-aged and elderly population, and it is extremely harmful to the
hearing status of patients. This study focused on the ultrastructural features of the middle ear mucosa of guinea
pigs with tympanosclerosis. In this study, the clinical auditory characteristics of guinea pigs before surgery and
the clinical effect of tympanic sclerosis in guinea pigs were analyzed. The guinea pigs with tympanic sclerosis
were analyzed, and the guinea pigs were tracked and recorded. After otoscope observation and recording, the
experimental animals were sacrificed at the prescribed time point. Five guinea pigs from each experimental
group were anesthetized with 10% medicine in the abdominal cavity, fixed on the back and exposed to the heart
of the guinea pig. The tissue was cut into the right ear, perfused through the left ventricle, washed with 100ml of
PBS solution, and then injected with 100ml of 4% formaldehyde phosphate buffer (PH=7). The head was
quickly cut off, parts of the bone were removed, and hearing tissue was eliminated. The tympanic membrane
and middle ear mucosa were peeled off with a surgical microscope. After pruning, all specimens were soaked in
5% formaldehyde overnight. Thirty ears accounted for 50.0% (30/60) of the detectable grade PAF. The grade of
PAF was 66.67±33.77ng/mL. In the serous middle ear exudate, the detectable grade of PAF is five, accounting
for about 50.0% (10/20), and the grade of PAF is six, about 33.22±25.11ng/ml. The total detectable rate of PAF
in all SOM guinea pigs was 66.66% (41/60). The results show that the SOM middle ear exudate contains PAF,
the detection rate of PAF in mucus middle ear exudate is higher than that of serous middle ear exudate, and the
grade of PAF is greatly improved than that of serous middle ear exudate. The spread of AB was significantly
improved in the tympanic sclerosis group, and injection of pneumococcal type 3 into the middle ear was
detrimental to hearing.

Published
2020-05-01