Expression of Inflammatory Factors in Prostate Cancer and Its Correlations with Various Clinicopathological Characteristics

  • Xiaonan Mu
Keywords: Inflammatory Factor NF-κB, Prostate Cancer Tissues, Protein Expression of HIF-1α, Specific Antigen of Prostate, Prostate Hyperplasia

Abstract

To analyze the expressions of inflammatory factors nuclear transcription factor-κB (NF-Κb) and
hypoxia-inducible factor-1α (HIF-1α) in the prostate cancer tissues and its correlations with various
clinicopathological characteristics, fresh prostate cancer tissues from 102 patients were taken as the
experimental group, prostate hyperplasia tissues from 58 patients were taken as the control group, and normal
tissues from 102 patients were taken as the blank group. Enzyme-linked immunosorbent assay (ELIA) was used
to determine the concentrations of NF-κB and HIF-1α in the three groups. The fluorescence quantitative
polymerase chain reaction (PCR) was used to detect the mRNA expression levels of NF-κB and HIF-1α in the
three groups. The immunohistochemistry was used to detect the protein expressions of NF-κB and HIF-1α in the
three groups. The variance was used to analyze the correlations between NF-κB, HIF-1α and clinicopathological
characteristics. The results showed that the mRNA expression (4.27  1.14), protein positive expression rate
(66.53%) and concentration (87.17  8.55pg/mL) of NF-κB in the experimental group were higher than those of
the control group and the blank group (P< 0.05); the mRNA expression (3.62  1.06), protein positive
expression rate (71.72%) and concentration (50.67  5.11ng/L) of HIF-1α in the experimental group were
significantly higher than those in the control group and blank group (P< 0.05); the positive expressions of
NF-κB and HIF-1α had obvious differences in age, and those in patient no younger than 50 years old were
higher than those in patient younger than 50 years old (P< 0.05); the positive expressions of NF-κB and HIF-1α
had observable differences in TNM stages, and those in patients at stage IV were significantly higher than those
in patients at stage II and III (P< 0.05); the positive expressions of NF-κB and HIF-1α in level of specific
antigen of prostate were greatly different, and those in patients with no less than 15ng/mL were higher than
those in patients with less than 15ng/mL (P< 0.05). In conclusion, the expression levels of inflammatory factors
NF-κB and HIF-1α in the prostate cancer tissues were higher than those in the prostatic hyperplasia tissues, and
were significantly positively correlated with TNM stage, age, and level of specific antigen of prostate.

Published
2020-05-01