Clinical Diagnosis of Bacterial Negative Pulmonary Tuberculosis by Microscopic Observation of Drug Sensitivity

  • Zhibin Liu
Keywords: Bacterial-Negative Tuberculosis, Microscopic Technology, Diagnosis

Abstract

To observe the clinical diagnostic effect of drug sensitivity technique, C-N acid-fast staining method and modified Roche medium culture method on bacterium-negative pulmonary tuberculosis by contrast microscopy.260 sputum samples of bacterium-negative tuberculosis and 20 sputum samples of other non-tuberculosis patients were collected from January 2017 to January 2020. The Mtb of sputum samples was detected by the anti-acid staining method, modified Roche medium culture method and microscopic observation of drug sensitivity technology. The detection of Mtb in one of the sputum samples under the three methods was recorded, and the mycobacteria obtained by the modified Roche medium culture method and microscopic observation of drug sensitivity technology were detected by one-layer analysis. The patients with bacterium-negative tuberculosis by microscopic observation of drug sensitivity technology and those with negative drug sensitivity technology by microscopic observation were followed up for 3 months. The follow-up results were used as diagnostic criteria, and the diagnostic effects of the three methods were compared and analyzed to evaluate the clinical diagnostic value of microscopic observation of drug sensitivity technology for bacterium-negative pulmonary tuberculosis. Twenty sputum samples from non-tuberculosis patients and 260 sputum samples from suspected tuberculosis patients were negative using the three detection methods. Among them, the positive results were 28.8% by ichthyl-nicotine acid-fast staining, 36.0% by modified Roche medium culture, and 42.8% by microscopic drug sensitivity technique. Among 102 positive sputum samples detected by C-N acid-fast staining method and modified Roche medium culture method, 97 were detected by microscopic observation of drug sensitivity technology; among 162 negative sputum samples detected by C-N acid-fast staining method and modified Roche medium culture method, 16 were positive by microscopic observation of drug sensitivity technology. If the diagnostic results were taken as the analysis criteria, the sensitivity and specificity of the drug sensitivity technique for microscopic observation were 63.4% and 97.8%, and the sensitivity and specificity of the arachidonic acid-fast staining method were 54.3% and 100.0%, respectively. The sensitivity and specificity of the modified Roche medium culture method were 43.3% and 100.0%, which were significantly different from the data of the three groups, and the difference was statistically significant (P < 0.05). The detection time of drug sensitivity technology by microscopy observation was 10 days, which was significantly less than the other two methods, and the difference was statistically significant (P < 0.05). Microscopic observation of drug sensitivity technology has more advantages than other detection methods in terms of sensitivity and detection time, and can make a more effective diagnosis of bacterial-negative pulmonary tuberculosis.

Published
2020-05-01