Effects of Rnase on fluorescence of CRY1 FISH Probed Native and Heat- Permeabilized Cryptosporidium Parvum Oocysts

  • Marín Adalberto
Keywords: Cryptosporidium Parvum, Parvum, rRNA, RNase, Species, Viability

Abstract

Quantitative analysis of bulk water for Cryptosporidium parvum oocysts currently involves four steps; concentration
of bulk water particulates, purification of oocysts from background detrital material (using floatation,
immunomagnetic separation or flow cytometry), immunofluorescent antibody (IFA) staining of the purified material,
and epifluorescence microscopy.

Published
2020-04-01