Experimental Study of Methylprednisolone on Spinal Cord Injury in Mice Using Two-photon Microscopy

  • Xiao Li
Keywords: Two-Photon Microscope, Methylprednisolone Treatment Effect, Spinal Cord Injury in Mice, in Vivo Imaging

Abstract

The spinal cord is part of the central nervous system. Spinal cord injury is prone to serious consequences and is
extremely harmful to individuals and society. Methylprednisolone can reduce secondary injury of spinal cord
injury through its anti-inflammatory and antioxidative effects, but the protective effect of methylprednisolone on
axons that compress spinal cord injury is not clear. In order to investigate the therapeutic effect of
methylprednisolone on spinal cord injury in mice, two-photon in vivo imaging technology was used in vivo to
observe the therapeutic effect of methylprednisolone on spinal cord injury in mice. In this paper, a control
experiment was used to divide the study mice into the sham operation group (group A), spinal cord injury group
(group B), and MP treatment group (group C). The two-photon microscope in vivo observation in this article
shows that the axon residual rate of group A is 1 at each time point, and the axon residual rate of group B and C
decreases within 48h after injury, and the axon residual rate of group C at 48h and 72h after injury is higher than
that of B. Group (P <0.05). The results of this study indicate that two-photon microscopy can detect the dynamic
changes of axons early after spinal cord compression injury. Early application of methylprednisolone in acute
spinal cord injury has a neuroprotective effect, and it can reduce secondary damage to the spinal nerve and slow
necrosis of the delayed nerve fibers.

Published
2020-02-01