Regular Exercise Affects the Expression of Monocarboxylate Transporters in Brains of AD Model Mice and Improves Cognitive Function

  • Weiguo Zhang
Keywords: Regular Exercise, AD Model Mice, Monocarboxylate Transporter, Cognitive Function

Abstract

Alzheimer's disease (AD) is the most common cause of Alzheimer's disease. It is a group of primary degenerative neurological diseases. The clinical manifestations are progressive deterioration of cognitive and memory functions, various neuropsychiatric symptoms and behavioral disorders. In order to explore the expression of monocarboxylate transporter in the brain of AD model mice, AD model mice (APP/PS1 double transgene) and wild-type mice of the same age were selected as the research subjects. The experimental subjects were subjected to regular aerobic exercise for 8 weeks, and the Y-maze behavioral test was used to investigate the learning and memory ability of the mice, and the protein expression levels were examined to observe the expression of MCTs. Firstly, the APP/PS1 transgenic mice were identified, and the propagated mice were subjected to DNA extraction, amplified by PCR, and separated by agarose gel electrophoresis. The banding range of the imaging analysis system was about 350 bp. This is the AD model mouse required for the experiment. Then, the Y-maze behavior detection method is used to judge the learning and memory function levels of the mice. The results showed that the AD model mice changed their behavior with age, and the learning and memory function decreased. Finally, the expression of monocarboxylate transporter was detected. It was found that the mice increased the expression of MCT1, MCT2 and MCT4 in the endothelial and hippocampus after a long period of regular aerobic exercise.

Published
2019-04-13